Ngwaahịa

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) bụ ihe eji arụ ọrụ nke 4-nitrophenyl (pNP) glycopyranoside maka nchọpụta α-galactosidase ọrụ.Ọnụ ọgụgụ pNP ewepụtara abawanyela nke ukwuu mgbe ejiri 4-Nitrophenyl α-D-galactpyranoside mee ihe dị ka ihe eji eme ihe.

Iji 4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPfromthe4-Nitrophenyla-D-galactopyranosidedị pụtara ihe dị elu karịa site na theotherpNP-glycopyranosidesatpH4.0 ebe a na-eme ihe omume n'ime akwụkwọ ntuziaka. yl (pNP) glycopyranosidesasartificial substrates na-egosi na a tọhapụrụ galactoseresidues sitere na4-Nitrophenyla-D-galactopyranosidemainlyatpH4.0, iethepHlekwa ebe a na-enweta nrịgo na-agbadata kacha.

Mkpụrụ nke α-D-galactosidase (α-D-Galactosidase).Na-ejikọta na E. coli lactose protein ebu.Ọkara maka α-D-galactose.

Usoro ngwa ngwa maka ịlele ọrụ lytic nke ọgwụ mgbochi megide yist na fungi ka emepụtara.Nnwale ahụ dabere na ntọhapụ nke enzyme intracellular, maltase (alfa-glucosidase).A tụrụ ọrụ maltase a tọhapụrụ na agba site na mmepụta p-nitrophenol sitere na p-nitrophenyl-alpha-D-glucopyranoside (PNPG).A tụrụ ọrụ lytic nke ogige mgbochi nje dị iche iche megide mkpụrụ ndụ yist ma ọ bụ spores nke filamentous fungi.Enwere ike ịchọpụta ọrụ mgbochi yist nke Lytic n'ime ntinye nkeji 20 na ogo 30 C megide Saccharomyces cerevisiae, Candida albicans, na Cryptococcus neoformans.Ọrụ mgbochi fungal Lytic pụtara mgbe awa 2 gachara na 30 Celsius C megide germinating spores nke Aspergillus niger na Botrytis cinerea.Mkpụrụ ndụ niile nke yist ma ọ bụ fungi emeghị ka mmiri mee PNPG zuru oke n'ime awa 3 na ogo 30 C iji nye mgbanwe agba nwere ike ịchọpụta.Achọpụtara ọrụ lytic nke enzymes (dịka ọmụmaatụ, Lyticase), ọgwụ nje (dịka, Amphotericin B), na ụdị nje nje na-emepụta nje site na iji nyocha ahụ.Emegharịrị nyocha mgbochi yiast ka ọ bụrụ usoro microtiter nke ọma 96.Nnwale abụọ ahụ nyere nchọpụta ngwa ngwa, dị nro na nke enwere ike imepụtaghachi nke lytic mgbochi yiast na mgbochi fungal.