Ngwaahịa

1.Anyị na-amụ mmetụta mgbochi nke isorhamnetin na ero tyrosinase site na mgbochi kinetics na simulation computational.Isorhamnetin mechiri emechi tyrosinase n'ụdị agwakọta na Ki=0.235±0.013 mM.Ntụle nke intrinsic na 1-anilinonaphthalene-8-sulfonate (ANS) -binding fluorescence gosiri na isorhamnetin emeghị ka mgbanwe dị ukwuu na nhazi ụlọ akwụkwọ nke tyrosinase.Iji nweta nghọta na usoro enweghị ọrụ, a na-agbakọ kinetics site na nha ogologo oge yana mmeghachi omume mkpụrụ na-aga n'ihu.Nsonaazụ gosiri na arụghị ọrụ nke isorhamnetin kpatara bụ mmeghachi omume mbụ na usoro biphasic.Iji nwetakwuo nghọta, anyị kpara nkata docking n'etiti tyrosinase na isorhamnetin.Simulation nwere ihe ịga nke ọma (ike njide maka Dock6.3: -32.58 kcal / mol, maka AutoDock4.2: -5.66 kcal / mol, na maka Fred2.2: -48.86 kcal / mol), na-atụ aro na isorhamnetin na-emekọrịta ihe na ọtụtụ ihe fọdụrụnụ, dị otú ahụ. dị ka HIS244 na MET280.Usoro a nke mmekọrịta tyrosinase amụma amụma yana nchikota kinetics dabere na ngwakọta flavanone nwere ike ịba uru na nyocha maka ndị na-egbochi tyrosinase eke.

2.Acid unfolding ụzọ nke conalbumin (CA), monomeric glycoprotein sitere na hen egg white, enyochala site na iji anya na nso-UV CD spectroscopy, intrinsic fluorescence emission, extrinsic fluorescence probe 1-anilino-8-napthalene sulfate (ANS) na ike ịgbasa ọkụ (DLS).Anyị na-ahụ mgbanwe pH dabere na usoro nke abụọ na nke atọ nke CA.O nwere usoro nke abụọ nke α-helical nke ala na pH 4.0 mana usoro mfu na pH 3.0.CA dị naanị dị ka steeti globule tupu a wụrụ awụ na steeti globule gbazere na ngwọta na pH 4.0 na pH 3.0, n'otu n'otu.Mmetụta nke pH na conformation na thermostability nke CA na-atụ aka na nguzogide okpomọkụ ya na pH na-anọpụ iche.Nsonaazụ DLS na-egosi na steeti MG dị ka kọmpat ụdị na ngwọta mmiri nwere hydrodynamic radii nke 4.7 nm.Nkwụsị nke tryptophan fluorescence site na acrylamide gara n'ihu na-akwado mmụkọ nke steeti etiti, nke ekpughere akụkụ ya, n'etiti steeti ala na agbasaghị.