Ngwaahịa

Chemifluorescence: Molecular luminol bụ molekul fluorescent kemịkalụ nke enwere ike ịtụgharị ka ọ bụrụ aminophthalic acid steeti nwere obi ụtọ n'ihu ụmụ irighiri hydrogen peroxide, nke na-ebunye ọkụ ọkụ.Hydrogen peroxide bụ ngwaahịa nke ọtụtụ mmeghachi omume biooxidative, yabụ ọ dị mfe ijikọ mmeghachi omume biooxidative ndị a na nchọpụta foto site na iwebata luminol.Dịka ọmụmaatụ, nyocha nke glucose oxidase / catalase nwere ike ịchọpụta mkpokọta hydrogen peroxide ma ọ bụ glucose na nlele, na oge nzaghachi bụ naanị 0.5s (usoro ike).Ijikọta ihe chemiluminescent na mmeghachi omume immunological, na ịkọwa mkpokọta nke ihe ndị na-alụso ọrịa ọgụ achọpụtara site na mmeghachi omume ọkụ, a na-akpọ teknụzụ immunoassay chemiluminescent.N'afọ 1976, Shmeder tụpụtara chemiluminescence immunoassay, na-eji luminol-H202 na nke ya ABEI dị ka usoro ngosipụta mmeghachi omume nchọpụta.Ka ọ dị ugbu a, luminol na usoro ya bụ ihe nrịbama a na-ejikarị na ngwa immunoassay chemiluminescence.N'okpuru ọnọdụ alkaline, nke microperoxidase catalyzed, enwere ike ịhapụ ọnụ ọgụgụ dị ukwuu nke photon.Enwere ike iji Luminol maka WesternBlot nke ọgwụ mgbochi HRP na nchọpụta nucleic acid hybridization nke nyocha akara HRP.A na-ejikwa ya na-achọpụta ntụpọ ọbara nke ọgbara ọhụrụ.Ngwakọta nke enwere ike iji dị ka akara chemiluminescent aghaghị izute ọnọdụ ndị a: mkpụrụ nke quantum nke luminescence dị elu;Njirimara physicochemical ya dabara na usoro a na-amụ;mmeghachi omume luminescent ya bụ nsonaazụ nke mmeghachi omume oxidation nke ihe luminescent;N'ime oke ịta ahụhụ ejiri n'ime na-adịghị egbu egbu maka ihe ndị dị ndụ.A na-ejikarị ụdị chemiluminescence reagents dị iche iche eme ihe: acridine ester, onye na-ahụ maka chemiluminescence nke a na-ejikarị eme ihe, ọ bụ ihe na-emepụta tricyclic organic, dị mfe oxidize, na mmeghachi omume oxidation anaghị achọ ihe na-eme ka ọ bụrụ onye na-emepụta ihe, na-ahapụ photons na 430nm;Luminol na isoluminol na usoro ha bụ ndị ọrụ chemiluminescent kacha tozuru oke.N'ihe dị ka 1964, Mgbe a na-akọ banyere chemiluminescence, luminol, isoluminol na ihe ndị ha na-emepụta na-eji chemiluminescence mee ihe.Immunoassays enwetala nsonaazụ ka mma.Nchọpụta luminescence: ogologo ikuku fluorescence kacha mma bụ 400nm (nchọpụta chemiluminescence na 60mmK2S2O8, 100mmK2CO3, pH11.5 ngwọta)

Chemiluminescence: Dị ka usoro nyocha nke na-emetụ n'ahụ, a na-eji chemiluminescence eme ihe n'ọtụtụ ebe iji chọpụta radicals free na mmeghachi omume metabolites na-emepụta na enzymes, mkpụrụ ndụ na ihe ndị dị ndụ.Enwere ike iji ọkụ dị iche iche na-emepụta site na ngwaahịa oxidation ha na metabolites.nchọpụta.A na-eji chemiluminescence eme ihe n'ọtụtụ ebe na nyocha na nyocha nke antioxidants, dị ka polyphenols, polysaccharides, flavonoids, na anthraquinones, n'ihi uche ya, ngwa ngwa, ọrụ dị mfe na ọnụ ala dị ala.Usoro chemiluminescence a na-ejikarị eme ihe maka mkpebi nke anions superoxide gụnyere xanthine oxidase-luminol, pyrogallol-luminol na dimethyl sulfoxide-luminol.Nke mbụ bụ usoro enzymatic, nke abụọ bụ usoro na-abụghị nke enzymatic.Usoro chemiluminescence maka mkpebi siri ike nke hydroxyl radicals na-agụnyekarị ọla kọpa sulfate-yist (ma ọ bụ ụmị ọkpụkpụ) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, ọla kọpa sulfate-o-phenanthroline-ascorbic acid-hydrogen. Sistemụ chemiluminescence peroxide 5, ferrous sulfate-luminol-hydrogen peroxide na ferrous sulfate-luminol, niile na-agụnye mmeghachi omume Fenton kpochapụwo iji mepụta radicals hydroxyl, wee wakpo onye na-ahụ maka luminescent iji mepụta chemiluminescence, nke enwere ike iji tụọ mwepụ nke ihe mgbakwunye. ọrụ nke reactive oxygen radicals.Usoro nnwale: jiri pyrogallol-luminol chemiluminescence usoro: luminol na-eme ka ọ bụrụ 0.05mol / L ngwọta ntinye uche na 0.05mol / L NaOH ngwọta, echekwara n'ebe gbara ọchịchịrị, na mmiri distilled okpukpu abụọ tupu iji Dilute na 1mmol / L ngwọta, jiri 1mmol/ L HCl iji mee 0.01mol / L ngwọta nke pyrogallol, chekwaa ya na refrjiraeto na 4 Celsius C, ma gbanye ya na mmiri distilled ugboro abụọ na 16 ugboro 6.25 × 10-4mol / L ngwọta tupu eji .0.05mol / L pH10.2Na2CO3-NaHCO3 buffer (nwere 0.1mmol / LEDTA) kwadebere tupu eji ya, wee gwakọta ya na 1mmol / L luminol na 2: 1 (mkpụrụkpụ mpịakọta) iji mepụta luminol na carbonate buffer tupu ngwakọta nnwale.N'oge nha, gbanye 10.0 μL samples nke dị iche iche iche (0, 0.08, 0.4, 2 na 10 mg / mL) n'ime luminescent cell (ya na sample ihe nchekwa dị ka njikwa), wee gbanye 6.25 × 10-4mol / L pyrogallol. 0.05 N'ikpeazụ, 0.94 mL nke ngwakọta nke luminol na carbonic acid buffer ka agbakwunyere ịmalite mmeghachi omume (30 ° C), a na-agụta ọkụ ọkụ na etiti oge nke 2 s, na mkpokọta luminescence jikọtara ike na 300 s.Ike n'azụ luminescence ike bụ luminescence mgbe etinyeghị pyrogallol.uru.Tụkwasị na nke ahụ, e nwere chemiluminescence, fluorescence spectroscopy na NBS-dichlorofluorescein chemiluminescence usoro maka mkpebi nke fluorescence chemiluminescence, wdg, uru ha kacha ukwuu bụ nnukwu uche.

Njirimara chemical: odo crystalline ntụ ntụ.A na-agbaze ngwa ngwa na lye, soluble n'ime acid dilute, ọ fọrọ nke nta ka ọ ghara ịsacha ya na mmiri, na mmanya na-egbu egbu.Ngwọta na-anọpụ iche ma ọ bụ ntakịrị acidic na-egosipụta ọkụ na-acha anụnụ anụnụ na-egbuke egbuke mgbe ekpughere ya na ìhè ultraviolet.Ebe mgbaze 329-332 ℃

Na-eji: Dị ka a chemical analysis reagent na egosi.Jiri reagent nchọpụta nyocha Chemiluminescence (dị ka mkpebi nke cations ọla ma ọ bụ ọbara)

Na-eji: Maka nyocha nke chemiluminescence, dị ka: cations metal, ọbara na glucocorticoids

Na-eji: luminescence ule: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; mgbe gbakwunyere H2O2) chemiluminescence reagent na egosi, nke a na-ejikarị na chemiluminescence analysis, dị ka metal cations, ọbara mgbochi, wdg.