3- [(3- Cholanidopropyl) dimethylamonio] -1 -propanesulfonate Cas: 75621-03-3 98%
Nọmba katalọgụ | XD90075 |
Aha ngwaahịa | 3-[(3-Cholanidopropyl) dimethylamonio]-1-propanesulfonate. |
CAS | 75621-03-3 |
Usoro ihe omimi | C32H58N2O7S |
Ibu molekụla | 614.88 |
Nkọwa nchekwa | Ambient |
Nkwekọrịta Koodu tarifu | 29242970 |
Nkọwapụta ngwaahịa
IR | Tụnyere ntụnyere aka |
Ọnwụ na ihicha | <2% |
TLC | Otu ntụpọ |
Nitrogen | 4.46 - 4.65 |
Ọdịdị | Ọcha ntụ ntụ |
Solubility (10% na H2O) | O doro anya na enweghị agba |
Ịrụ ọrụ (0.1M na H2O) | <10us/cm |
Nyochaa HPLC | > 98% |
UV (5% na H2O) A290nm | <0.5 |
UV (5% na H2O) A400nm | <0.02 |
Ọdịnaya mgbaze (nke GC) Dimethylformamide | <2.0% |
3-[3- (cholamidopropyl) dimethylamino] propanesulfonic acid n'ime nnu ojiji na njikọ usoro.
Ọrụ ndu: CHAPS, ihe ewepụtara nke Cholicacid, bụ ncha zwitterionic nke na-eme ka protein dị n'ime ahụ solubilize.A na-eji CHAPS kwadoo ogige protein-DNA dị iche iche ma nwee ike ijigide ọrụ biochemical nke protein na ngwọta.
Na-eji: CHAPS bụ ncha zwitterionic na-adịghị emebi emebi maka akpụkpọ ahụ biochemistry.Enwere ike iji solubilize protein akpụkpọ anụ ma mebie mmekọrịta protein-protein.CHAPS nwere obere molecular arọ (6,150) yana mkpokọta micelle dị oke egwu (6-10 mM) yabụ enwere ike wepu ya na nlele site na dialysis.Ọ dịkwa mma maka solubilizing protein na isoelectric elekwasị anya na 2D electrophoresis.A na-ejikarị CHAPS eme ihe na nleba anya isoelectric nke ala ala (urea na-enweghị urea) wee hụ na ọ na-enye mkpebi dị mma maka ụfọdụ nkwadebe subcellular na protein osisi.A na-ejikarị mkpokọta n'etiti 2-4% (w/v) maka gels na-elekwasị anya.
Na-eji: Ncha zwitterionic na-abụghị denaturing maka membrane biochemistry, nke enwere ike iji mee ka proteins na-edozi ahụ ma mebie mmekọrịta protein-protein;dịkwa mma maka solubilizing protein na isoelectric na-elekwasị anya na akụkụ abụọ nke electrophoresis;a na-ejikarị na-abụghị denaturing (enweghị urea) isoelectric elekwasị anya;Mwepụta RNA na DNA, enweghị ọrụ RNase na DNA
Na-eji: Amphoteric surfactant, CHAPS bụ ihe na-adịghị denaturing zwitterionic nsacha, protein lysate maka solubilizing protein protein na lysing mmekọrịta n'etiti protein na proteins.