Ngwaahịa

N'iji nitrogen eme ihe nke ọma na ehi dị mkpa iji nweta mmepụta ehi na-adịgide adịgide.Dị ka purines na pyrimidines (PP) bụ akụkụ dị mma nke rumen nitrogen, nghọta ka mma nke absorption na intermediary metabolism nke PP dị mkpa.Ọrụ dị ugbu a na-akọwa mmepe na nkwenye nke usoro dị nro na nke akọwapụtara maka ikpebi otu oge nke 20 purines (adenine, guanine, guanosine, inosine, 2'-deoxyguanosine, 2'-deoxyinosine, xanthine, hypoxanthine), pyrimidines (cytosine, thymine, uracil, cytidine, uridine, thymidine, 2'-deoxyuridine), na ngwaahịa mmebi ha (uric acid, allantoin, β-alanine, β-ureidopropionic acid, β-aminoisobutyric acid) na plasma ọbara nke ehi mmiri ara ehi.Usoro dabere na chromatography mmiri mmiri dị elu jikọtara ya na electrospray ionization tandem mass spectrometry (LC-MS/MS) jikọtara ya na ụkpụrụ nhazi nke matriks dabara na nke ọ bụla yana ogige ntụnyere ahapụrụ iche.Ntụle ọnụọgụgụ bu ụzọ tupu usoro ọgwụgwọ ọhụụ nke gụnyere mmiri ozuzo ethanol, filtration, evaporation na reconstitution.A na-enyocha usoro maka nkewa na nchọpụta n'oge nyocha LC-MS/MS.Ekwenyere na iji usoro nhazi log-calibration karịa usoro nhazi ahịrị mere ka CV dị ala na enweghị ule dabara adaba gosipụtara nlọghachi ahịrị ahịrị na-eju afọ.Usoro a na-ekpuchi ọnụọgụ ịta ahụhụ maka metabolite ọ bụla dịka nke ahụ dị na nlele n'ezie, dịka guanine: 0.10-5.0 μmol/L, na allantoin: 120-500 μmol/L.CV% maka ọnụọgụ ọnụọgụ ahọpụtara nọ n'okpuru 25%.Usoro a nwere ezigbo nkwughachi (CV%≤25%) yana nzizi etiti (CV%≤25%) yana ezigbo mgbake (91-107%).Metabolites niile gosipụtara ezigbo nkwụsi ike ogologo oge yana ezigbo nkwụsi ike n'ime ọsọ (CV%≤10%).Ahụrụ ogo dị iche iche nke mmetụta matriks zuru oke na plasma, mmamịrị na mmiri ara ehi.Mkpebi nke mmetụta matrix ikwu na-egosi na usoro ahụ dabara adaba maka ihe fọrọ nke nta ka ọ bụrụ enyochala metabolites PP niile na plasma nke sitere na akwara akwara na akwara imeju, imeju na gastrosplenic veins na, ma ewezuga ole na ole, yana maka ụdị ndị ọzọ dị ka ọkụkọ, ezi, mink, mmadụ na oke.