Ngwaahịa

Anyị na-ezute na mbụ usoro nhazi ebe dihydrotestosterone (DHT) gosipụtara mmetụta mgbochi ya na protein parathyroid hormone metụtara parathyroid (PTHrP) gene repression site na estrogen receptor (ER) α, ma ọ bụghị androgen receptor (AR), na ọrịa cancer ara MCF-7 sel. .N'ebe a, anyị nyochara ma mmekọrịta dị otú ahụ aberrant ligand-nuklear receptor (NR) dị na sel LNCaP cancer prostate.Nke mbụ, anyị kwadoro na mkpụrụ ndụ LNCaP gosipụtara nnukwu AR na ọkwa ERα/β ma ọ bụ progesterone nnabata.Ma nbibi nke PTHrP na ịgbalite prostate-specific antigen genes ka a hụrụ mgbe nchịkwa onwe ya nke 17β-estradiol (E2), DHT, ma ọ bụ R5020.N'ikwekọ n'echiche ahụ na LNCaP AR tụfuru nkọwa nke ligand ya n'ihi ngbanwe (Thr-Ala877), nnwale nke siRNA na-elekwasị anya na NR dị iche iche gosiri na AR na-achịkwa ọrụ nke onye ogbugbo nke ụkpụrụ na-adabere na hormone, nke na-ejikọta ya mgbe niile. nwere translocatio nuklia n nke mutant AR a.Nyocha microarray nke usoro mkpụrụ ndụ ihe nketa site na DHT, E2, ma ọ bụ R5020 gosipụtara na ihe karịrị ọkara nke mkpụrụ ndụ ihe nketa nke dị n'okpuru AR (Thr-Ala877) gbakọtara na sel LNCaP.N'ime mmasị pụrụ iche, anyị chọpụtara na AR (ụdị anụ ọhịa [wt]) na AR (Thr-Ala877) bụkwa ndị na-ahụ maka mmekọrịta E2-AR.Nnwale microscopy fluorescence gosipụtara na EGFP-AR (wt) na EGFP-AR (Thr-Ala877) bụ naanị mpaghara n'ime oghere mgbe ọgwụgwọ E2 ma ọ bụ DHT gasịrị.Ọzọkwa, nyocha ndị nta akụkọ kpughere na ụfọdụ mkpụrụ ndụ kansa ndị ọzọ gosipụtara ihe na-adịghị mma E2-AR (wt) nke na-egosi na sel LNCaP.N'ebe a, anyị na-ekwupụta ọnụnọ nke mmekọrịta dị n'etiti wt AR na E2 n'ime ụfọdụ mkpụrụ ndụ kansa na-enwe mmetụta nke homonụ.