Ngwaahịa

1.A ga-akọwa usoro ọhụrụ maka nkwalite ninhydrin ma ọ bụ 1,8-diazafluoren-9-one (DFO) -mkpụrụ mkpịsị aka latent mebere na akwụkwọ ọkụ.Ọtụtụ ihe na-ekpo ọkụ nke akwụkwọ ọkụ na-agba ọchịchịrị mgbe ejiri DFO ma ọ bụ ninhydrin petroleum ether (NPB) agwọ ya.Mmetụta a na-ebelata ọdịiche dị n'etiti akara mkpịsị aka emepụtara na ndabere.Usoro ọhụrụ a kọwara na-ebelata ntụpọ ọchịchịrị a na-ewepụghị oyi akwa thermosensitive na akụkụ nke akara mkpịsị aka emepụtara, dị ka ọ na-eme na ịsacha acetone.Site na usoro ọhụrụ ahụ, akara mkpịsị aka emepụtara na-apụta n'ahịrị dị nkọ na ọdịiche dị elu.Emere ule dị ukwuu, na-eduga na ngwọta ọrụ kachasị mma, nke na-ebu akwụkwọ ahụ na obere kemịkalụ, dị ọnụ ala, ma na-enyere aka ịgwọ nnukwu akwụkwọ n'ime obere oge.Ngwọta na-arụ ọrụ nwere nke a na-ere ahịa, nke na-adịghị agbanwe agbanwe, ogige nitrogenous organic na enwere ike iji ya dị ka ngwa NPB ngwọta site n'itinye.

2. Akwụkwọ a na-akọwa ọmụmụ nke mmeghachi omume n'etiti 1, 8-diazafluoren-9-one (DFO) na amino acid L-alanine na mmanya methyl.Enyere ọmụrụ nwa pụrụ iche maka ọrụ mgbaze nwere ike ime nke yiri ka ọ na-emeghachi omume na DFO iji mepụta hemiketal nke bụ ụdị mmeghachi omume.A na-atụpụta ụzọ mmeghachi omume nwere ike dabere na data anakọtara ugbu a.Achọpụtara etiti mmeghachi omume na ngwaahịa site na ụzọ dị iche iche spectroscopic gụnyere mass spectrometry (MS), spectroscopy magnetik resonance (NMR), na X-ray crystallography.