Otu nje bacteria entomopathogenic, Xenorhabdus nematophila, na-ebute ihe mgbochi nke ụmụ ahụhụ ezubere iche site na igbochi ọrụ phospholipase A(2) (PLA(2)).N'oge na-adịbeghị anya, a chọpụtara mkpụrụ ndụ ihe nketa nke PLA(2) nke na-alụso ọrịa ọgụ site na ebe ntụ ọka uhie, Tribolium castaneum.Ọmụmụ ihe a mechiri mkpụrụ ndụ PLA (2) na vector okwu nje iji mepụta enzyme recombinant.The recombinant T. castaneum PLA (2) (TcPLA (2)) gosipụtara njirimara enzyme ọrụ ya na ntinye ntinye, pH, na okpomọkụ gburugburu.Njirimara biochemical ya dabara na ụdị nzuzo nke PLA (2) (sPLA (2) n'ihi na egbochiri ọrụ ya site na dithiothreitol (onye na-ebelata mkpokọta disulfide bond) na bromophenacyl bromide (onye na-egbochi spLA (2) kpọmkwem) mana ọ bụghị site na methylarachidonyl. fluorophosphonate (ụdị cytosolic akọwapụtara nke PLA (2)).Broth omenala X. nematophila nwere PLA(2) ihe na-egbochi ihe (s), nke kachasị na mgbasa ozi enwetara n'oge uto nje bacteria na-eguzo.Ihe na-egbochi PLA(2) bụ ihe na-egbochi okpomọkụ ma wepụta ya na akụkụ abụọ nke mmiri mmiri na nke organic.Mmetụta nke PLA (2) -nchịkọta ihe mgbochi na mgbochi immunosuppression nke T. castaneum yiri nke ahụ sitere na mgbochi nke TcPLA (2) mkpụrụ ndụ ihe nketa site na nnyonye anya RNA.
