Ngwaahịa

Ọrịa imeju na-adịghị egbu egbu (NAFLD) nwere ụdị ọrịa dịgasị iche iche sitere na steatosis dị nro nke ejiri njigide nke lipids n'ime sel imeju na steatohepatitis (NASH) na-egosi nchịkọta abụba, mbufụt, balloon na mmebi nke hepatocytes, na fibrosis.N'ikpeazụ, steatohepatitis nwere ike ịkpata ọrịa cirrhosis imeju na hepatocellular carcinoma. N'ime ọmụmụ ihe a, anyị enyochala ụdị òké atọ dị iche iche, A/J, C57BL/6J, na PWD/PhJ, nke na-egosi ọkwa dị iche iche nke steatohepatitis mgbe a na-enye 3,5-diethoxycarbonyl. -1,4-dihydrocollidine (DDC) nwere nri.A na-etinye nyocha okwu mkpụrụ ndụ RNA-Seq, nyocha protein na profaịlụ nke metabolic iji chọpụta mkpụrụ ndụ ihe nketa/protein dị iche iche na ọkwa metabolite nke ihe nlele imeju òké n'elu ọgwụgwọ DDC.Nyocha ụzọ gosipụtara mgbanwe nke arachidonic acid (AA) na S-adenosylmethionine (SAMe) metabolism na ụzọ ndị ọzọ.Iji ghọta mgbanwe metabolic nke arachidonic aci d metabolism n'iburu n'uche ngosipụta nke ọrịa, e mepụtara ụdị kinetic nke ụzọ a ma kwalite ya dabere na ọkwa metabolite.N'ikpeazụ, a na-eji ihe nlereanya ahụ na-amụ na mmetụta silico nke nwere ike ịchọta ọgwụ maka steatohepatitis. Anyị chọpụtara AA / eicosanoid metabolism dị ka nnukwu mgbagwoju anya na ụmụ oke ndị DDC na-ebute site na iji ngwakọta nke nnwale na n'ụzọ silico.Nyocha anyị nke ụzọ AA / eicosanoid metabolic ụzọ na-atụ aro na 5-hydroxyeicosatetraenoic acid (5-HETE), 15-hydroxyeicosatetraenoic acid (15-HETE) na prostaglandin D2 (PGD2) na-akpaghasị na ụmụ oke DDC.Anyị na-egosipụta n'ihu na enwere ike iji ihe atụ dị ike mee ihe maka amụma qualitative nke mgbanwe metabolic dabere na data transcriptomics na ọnọdụ metụtara ọrịa.Ọzọkwa, a chọpụtara na metabolism SAME dị ka ọ na-agbagwoju anya n'ihi ọgwụgwọ DDC.Ọtụtụ mkpụrụ ndụ ihe nketa yana ụfọdụ metabolites nke modul a na-egosi ọdịiche dị n'etiti A/J na C57BL/6J n'otu aka na PWD/PhJ n'aka nke ọzọ.